generation run 2026-04-26
64 min completedmodel pipeline-v5plan not recordedrun/96713cc7…Selection
Verification
- record
- seen / partial
- working
The model's own caveats
none raised on this run
Reproduce
- inputs
- 30 source keys (listed under Sources)
- plan
- not recorded — template version, prompt hash and git SHA are written from 2026-09 onward
- model
- pipeline-v5
- streams
- none — agent transcripts are persisted from 2026-09 onward
23 searches — query, source, results, what was kept and why
| # | source | query | seen | kept | rationale |
|---|---|---|---|---|---|
| 1 | PubMed | 2C-T-7 serotonin receptor binding affinity Ki | 1 | 1 | — |
| 2 | PubChem | 2C-T-7 | 1 | 1 | Primary chemical identity verification |
| 3 | PubMed · absence | 2C-T-7 forensic toxicology death fatality | 0 | — | — |
| 4 | PubMed | 2,5-dimethoxy-4-propylthiophenethylamine receptor pharmacology | 8 | 5 | — |
| 5 | PubMed | 2C-T-7 phenethylamine pharmacology | 10 | 7 | — |
| 6 | ClinicalTrials.gov · absence | 2C-T-7 | 0 | — | — |
| 7 | OpenAlex | 2C-T-7 psychedelic phenethylamine | 10 | 0 | — |
| 8 | OpenAlex | 2C-T-7 propylthio phenethylamine psychedelic drug | 10 | 2 | — |
| 9 | PubMed · absence | 2C-T-7 toxicity overdose death case report | 0 | — | Will retry with alternative search terms |
| 10 | Federal Register / DEA | 2C-T-7 propylthiophenethylamine schedule I | 3 | 2 | — |
| 11 | PubMed | PMID 28720478 | 1 | 1 | — |
| 12 | WebSearch | 2C-T-7 overdose death fatality case report Beautiful Blue Mystic | 10 | 0 | — |
| 13 | PubChem | CID 24728635 | 1 | 1 | — |
| 14 | PubMed | 2C-T-7 urine metabolites mass spectrometry GC-MS HPLC detection | 1 | 1 | — |
| 15 | PubMed · absence | 2C-T-7 binding 5-HT2A 5-HT2C TAAR1 Ki nM in vitro radioligand | 0 | — | — |
| 16 | WebSearch | 2C-T-7 legal status UK Germany Netherlands Australia Canada international scheduling | 10 | 2 | — |
| 17 | WebSearch | 2C-T-7 Shulgin PIHKAL history synthesis discovery 1986 | 10 | 1 | — |
| 18 | PubMed/Crossref | DOI 10.1002/jms.784 / PMID 15643651 | 1 | 1 | — |
| 19 | PubMed | PMID 17337633 | 1 | 1 | — |
| 20 | WebSearch | 2C-T-7 Casey Hardison MAPS 2000 subjective bioassay survey | 10 | 1 | — |
| 21 | WebSearch/Erowid | 2C-T-7 insufflated vs oral effects comparison | 10 | 1 | — |
| 22 | PubMed · absence | 2C-B 2C-I 2C-E drug intoxication emergency department hospital clinical 2C phenethylamine acute | 0 | — | — |
| 23 | Multiple | Chemical identity verification | 2 | — | — |
70 claims by section — every cited sentence, checked against its source
safety17 · 11 ok
Claim in safety — inconclusiveinconclusiveworking →
Three fatalities associated with 2C-T-7 were documented in the United States between late 2000 and April 2001partially supported
One death from mono-intoxication documented in USA. pmid:16272191
One confirmed mono-intoxication death occurred following insufflation of an excessive dosesupported
One death from mono-intoxication documented in USA. pmid:16272191
Claim in safety — inconclusiveinconclusiveworking →
Anxiety and panic attacks are documented at higher doses in both Shulgin's reports and community surveyssupported
Adverse: hallucinations, nausea, anxiety, panic, paranoid ideation. pmid:16272191
Paranoid ideation has been notedsupported
Adverse: hallucinations, nausea, anxiety, panic, paranoid ideation. pmid:16272191
DOI-tolerant mice show insurmountable cross-tolerance to 2C-T-7 — increasing the dose cannot overcome the tolerance — indicating shared receptor mechanismssupported
DOI-tolerant mice showed insurmountable cross-tolerance to 2C-T-7. jpet.114.219337
Phenethylamine hallucinogens develop tolerance more rapidly than tryptamine hallucinogens.supported
Phenethylamine-derived hallucinogens develop tolerance faster than tryptamines. jpet.114.219337
Binding data show no significant activity at monoamine transporters (DAT, SERT, NET >4,000 nM), suggesting negligible direct engagement of dopaminergic reward circuitrysupported
no monoamine transporter activity (Ki > 4000 nM) j.neuropharm.2017.07.012
2C-T-7 is primarily metabolized by MAO-A and MAO-Bsupported
MAO-A and MAO-B are major enzymes for deamination of 2C-T-7. j.bcp.2006.09.033
Concurrent serotonergic burden from both 2C-T-7's direct receptor agonism and its endogenous MAO inhibition creates a severe serotonin toxicity risk.supported
17 2C-series drugs including 2C-T-7 tested for MAO-A and MAO-B inhibition. IC50 values for MAO-A ranged 10-125 uM, MAO-B 1.7-180 uM. dta.2494
Concurrent serotonergic burden from both 2C-T-7's direct receptor agonism and its endogenous MAO inhibition creates a severe serotonin toxicity risk.supported
2Cs likely susceptible to drug-drug interactions with MAO inhibitors. j.bcp.2006.09.033
Claim in safety — inconclusiveinconclusiveworking →
CYP2D6 plays a minor role in 2C-T-7 metabolismsupported
CYP2D6 also involved but to small extent. j.bcp.2006.09.033
Claim in safety — inconclusiveinconclusiveworking →
Claim in safety — inconclusiveinconclusiveworking →
Claim in safety — inconclusiveinconclusiveworking →
pharmacology13 · 9 ok
CYP2D6 contributes to a minor extent; other cytochrome P450 isoforms play negligible roles.supported
MAO-A and MAO-B are major enzymes for deamination of 2C-T-7. CYP2D6 also involved but to small extent. j.bcp.2006.09.033
The metabolic fate of 2C-T-7 has been characterized in rat urine:supported
Metabolism of 2C-T-7 in rat urine by GC/MS. jms.784
Tolerance develops rapidly through 5-HT₂A receptor downregulation, with bidirectional cross-tolerance to other phenethylamine hallucinogens demonstrated in micesupported
Tolerance develops to daily 2C-T-7 HTR in mice. DOI-tolerant mice showed insurmountable cross-tolerance to 2C-T-7. jpet.114.219337
Claim in pharmacology — inconclusiveinconclusiveworking →
Claim in pharmacology — inconclusiveinconclusiveworking →
Claim in pharmacology — inconclusiveinconclusiveworking →
Claim in pharmacology — inconclusiveinconclusiveworking →
In the head-twitch response (HTR) assay — a validated behavioural proxy for 5-HT₂A agonism — 2C-T-7 produces robust responses in both mice and rats.supported
SAR study of 4-thio-substituted phenylalkylamines including 2C-T-2 and 2C-T-7 using HTR assay. Findings support classification of 2C-T compounds as psychedelic drugs acting at 5-HT2A receptors. s00213-022-06279-2
Phenylisopropylamines (the amphetamine homologs) demonstrate greater efficacy than their phenethylamine counterparts at 5-HT₂A.supported
Phenylisopropylamines more efficacious than phenethylamines at 5-HT2A. pmid:17337633
Testing of 17 2C-series drugs including 2C-T-7 using heterologously expressed enzymes yielded class-range IC₅₀ values of 10–125 µM for MAO-A (7 compounds with determinable IC₅₀) and 1.7–180 µM for MAO-B (9 compounds with determinable IC₅₀)supported
17 2C-series drugs including 2C-T-7 tested for MAO-A and MAO-B inhibition. IC50 values for MAO-A ranged 10-125 uM, MAO-B 1.7-180 uM. dta.2494
This dual mechanism (5-HT₂A agonism combined with MAO inhibition) creates a serotonin syndrome risk profile not shared by all psychedelic phenethylamines.{{/Warning}}supported
17 2C-series drugs including 2C-T-7 tested for MAO-A and MAO-B inhibition. IC50 values for MAO-A ranged 10-125 uM, MAO-B 1.7-180 uM. dta.2494
The principal urinary metabolite in rat is β-hydroxylated-N-acetylated-sulfoxide — a profile distinct from 2c-t-2 (N-acetylated-sulfoxide) and 2C-T-4 (S-methylated-N-acetylated-sulfoxide), demonstrating metabolite specificity within the 2C-T series despite shared structural features.supported
For 2C-T-7, principal metabolite was beta-hydroxylated-N-acetylated-sulfoxide. bpb.b15-00953
For the structurally related 2C-T-7-FLY (a rigidified analog), the principal metabolic transformations are hydroxylation and N-acetylation, with CYP2D6, CYP3A4, FMO3, and NAT1/NAT2 identified as participating enzymessupported
Main metabolic steps: hydroxylation and N-acetylation. CYP2D6, 3A4, FMO3 and NAT1/NAT2 involved. dta.2675
summary8 · 5 ok
Brief recreational circulation in 2000–2001 produced three associated fatalities in the United States, prompting DEA temporary Schedule I placement in September 2002 and permanent scheduling in March 2004.partially supported
One death from mono-intoxication documented in USA. pmid:16272191
2C-T-7 acts as a partial agonist at 5-HT₂A receptors with class-range Ki of 1–54 nMsupported
5-HT2A Ki 1-54 nM... partial agonists at 5-HT2A/2B j.neuropharm.2017.07.012
Claim in summary — inconclusiveinconclusiveworking →
Claim in summary — inconclusiveinconclusiveworking →
Claim in summary — inconclusiveinconclusiveworking →
Secondary activity includes partial agonism at 5-HT₂C, agonism at rat TAAR1 (not human TAAR1), and MAO-A/MAO-B inhibition at micromolar concentrations.supported
IC50 values for MAO-A ranged 10-125 uM, MAO-B 1.7-180 uM. dta.2494
The compound's dual action as a 5-HT₂A agonist and MAO inhibitor creates a pronounced serotonin syndrome risk when combined with serotonergic agentssupported
17 2C-series drugs including 2C-T-7 tested for MAO-A and MAO-B inhibition. IC50 values for MAO-A ranged 10-125 uM, MAO-B 1.7-180 uM. dta.2494
The compound's dual action as a 5-HT₂A agonist and MAO inhibitor creates a pronounced serotonin syndrome risk when combined with serotonergic agentssupported
2Cs likely susceptible to drug-drug interactions with MAO inhibitors. j.bcp.2006.09.033
comparative-pharmacology7 · 4 ok
Claim in comparative-pharmacology — not_supportednot supportedworking →
The propyl chain at the 4-thio position yields the highest in vitro 5-HT₂A activation potency among the 2C-T homologs (class Ki range 1–54 nM), compared to broader ranges for other chain lengths (2–56 nM)supported
4-propyl analog produced highest potencies at 5-HT2A (1-9 nM) s41380-025-03325-1
The principal urinary metabolite of 2C-T-7 (β-hydroxylated-N-acetylated-sulfoxide) differs from those of 2c-t-2 and 2C-T-4, indicating that even minor structural variations within the series alter metabolic fate.supported
Rat urinary metabolism of 2C-T-2, 2C-T-4, 2C-T-7 after 10 mg/kg oral dosing. For 2C-T-7, principal metabolite was beta-hydroxylated-N-acetylated-sulfoxide. bpb.b15-00953
Phenethylamine-derived hallucinogens, including 2C-T-7, develop tolerance to the head-twitch response more rapidly than tryptamine-derived hallucinogenssupported
Phenethylamine-derived hallucinogens develop tolerance faster than tryptamines. jpet.114.219337
Claim in comparative-pharmacology — inconclusiveinconclusiveworking →
This is a consistent finding across the 2,5-dimethoxy series: the alpha-methyl group that distinguishes amphetamines from phenethylamines enhances 5-HT₂A functional efficacysupported
Phenylisopropylamines more efficacious than phenethylamines at 5-HT2A. pmid:17337633
Claim in comparative-pharmacology — inconclusiveinconclusiveworking →
pharmacokinetic-detail7 · 5 ok
The dominance of MAO over CYP metabolism has direct pharmacological implications: co-administration of MAO inhibitors would be expected to substantially increase plasma levels and prolong the duration of action, while CYP2D6 inhibitors (fluoxetine, paroxetine, bupropion, quinidine) would produce a comparatively minor effect on elimination.supported
MAO-A and MAO-B are major enzymes for deamination of 2C-T-7. CYP2D6 also involved but to small extent. 2Cs likely susceptible to drug-drug interactions with MAO inhibitors. j.bcp.2006.09.033
Theobald, Fehn, and Maurer identified four primary metabolic pathways in rat urine via GC-MSsupported
Metabolism of 2C-T-7 in rat urine by GC/MS. Key pathways: hydroxylation of propyl side chain + N-acetylation + sulfoxidation; deamination to acid or alcohol; S-dealkylation + N-acetylation + S-methylation + sulfoxidation (minor). jms.784
The principal urinary metabolite was confirmed as β-hydroxylated-N-acetylated-sulfoxide — a profile specific to 2C-T-7 within the 2C-T series, differing from 2c-t-2 (N-acetylated-sulfoxide) and 2C-T-4 (S-methylated-N-acetylated-sulfoxide).supported
Rat urinary metabolism of 2C-T-2, 2C-T-4, 2C-T-7 after 10 mg/kg oral dosing. For 2C-T-7, principal metabolite was beta-hydroxylated-N-acetylated-sulfoxide. bpb.b15-00953
2C-T-7 is detectable in blood and urine by GC-EI-MS and HPLC-electrospray-MSsupported
GC-MS screening method for designer tryptamines/phenethylamines including 2C-T-7. LOD 5-10 ng/mL in blood; LC-MS range 25-1500 ng/mL in urine. pmid:15516287
Claim in pharmacokinetic-detail — inconclusiveinconclusiveworking →
Claim in pharmacokinetic-detail — not_supportednot supportedworking →
Comprehensive analytical reviews of detection methods for 2,5-dimethoxy-phenethylamines have been publishedsupported
Review of analytical methods for quantification of 2,5-dimethoxy-amphetamines and phenethylamines in biological matrices. s11419-022-00638-6
history5 · 2 ok
Peer-reviewed review confirmed one death related to mono-intoxication with 2C-T-7 in the United States and noted very low street-level availability in the European Union at that time.supported
One death from mono-intoxication documented in USA. Low street availability in EU. pmid:16272191
Claim in history — inconclusiveinconclusiveworking →
Claim in history — inconclusiveinconclusiveworking →
Three deaths associated with 2C-T-7 occurred in the United States between late 2000 and April 2001, precipitating regulatory action:partially supported
One death from mono-intoxication documented in USA. pmid:16272191
Claim in history — inconclusiveinconclusiveworking →
neuroscience5 · 5 ok
Based on its confirmed 5-HT₂A agonism, the following neural mechanisms are inferred from the broader serotonergic psychedelic literature.supported
Schild analysis confirms predominant 5-HT2A receptor role for DOM, 2C-T-7, and DPT discriminative stimulus effects in rhesus monkeys. jpet.108.145458
Based on its confirmed 5-HT₂A agonism, the following neural mechanisms are inferred from the broader serotonergic psychedelic literature.supported
Confirms 5-HT2A receptor mediation. pmid:17993605
Based on its confirmed 5-HT₂A agonism, the following neural mechanisms are inferred from the broader serotonergic psychedelic literature.supported
Confirms 5-HT2A mechanism. jpet.109.163451
2C-T-7's additional activity at 5-HT₂C and its MAO inhibitory properties may modulate these effects in ways distinct from pure 5-HT₂A agonistssupported
17 2C-series drugs including 2C-T-7 tested for MAO-A and MAO-B inhibition. dta.2494
The compound's in vitro neurotoxicity profile — concentration-dependent mitochondrial membrane depolarization, ATP depletion, disrupted calcium regulation, and glutathione reduction in differentiated SH-SY5Y cells — raises questions about potential neuronal stress at pharmacologically relevant concentrationssupported
Concentration-dependent cytotoxicity. Mitochondrial membrane depolarization, lowered ATP, calcium dysregulation for 2C-T-7. jox14020044
receptor-pharmacology4 · 3 ok
The primary in vitro receptor characterization of the 2C-T drug class comes from Luethi and colleagues, who profiled 4-thio-substituted phenethylamines including 2C-T-7 across multiple receptor targets:supported
4-Thio-substituted phenethylamines (2C-T drugs) are potent psychedelics. Characterized monoamine receptor and transporter interaction profiles. j.neuropharm.2017.07.012
Claim in receptor-pharmacology — inconclusiveinconclusiveworking →
Among 2C-X phenethylamines, lipophilicity correlates with efficacy in both β-arrestin2 and miniGα_q recruitment assays at 5-HT₂Asupported
beta-arrestin2 vs miniGalphaq recruitment. Lipophilicity of 2C-X phenethylamines correlates with efficacy acschemneuro.3c00267
Extension beyond propyl reduces in vivo potency, partly attributable to pharmacokinetic limitations rather than receptor affinity alone.supported
extending chain beyond propyl reduces in vivo potency partly due to pharmacokinetic parameters. s41380-025-03325-1
chemistry2 · 1 ok
The 4-thioalkyl substitution pattern was systematically explored by Shulgin, who found the propyl chain length optimal for psychoactive potency — a finding later confirmed by receptor binding studies showing that propyl analogs achieve the highest 5-HT₂A activation potencies (1–9 nM range) within the 4-alkyl-2,5-dimethoxy series.supported
4-propyl analog produced highest potencies at 5-HT2A (1-9 nM) s41380-025-03325-1
Claim in chemistry — inconclusiveinconclusiveworking →